Lipogenesis in rabbit isolated fat-cells
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چکیده
منابع مشابه
Lipogenesis in rat and guinea-pig isolated epididymal fat-cells.
Fat-cells were prepared from rat and guinea-pig epididymal adipose tissue and compared on the basis of the intracellular distributions and activities of enzymes and with respect to their utilization of various U-(14)C-labelled substrates for lipogenesis. 1. Compared with the rat, guinea-pig extramitochondrial enzyme activities differed in that aconitate hydratase, alanine aminotransferase, ATP-...
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1. Isolated rat fat cells were incubated at 37 degrees with [U-14C]-glucose 0.55 mM and 125I-labeled insulin. The amount of receptor-bound 125I-labeled insulin and the rate of insulin-induced 14C-lipid synthesis were assessed during association and dissociation of 125I-labeled insulin. 2. The rate of 14C-lipid synthesis was constant from zero time in the absence of insulin and in the presence o...
متن کاملEffects of prolonged incubation and cell concentration on lipogenesis from glucose in isolated human omental fat cells.
The behavior of human omental fat cells in vitro has been examined in order to define conditions under which glucose is converted to glyceride-glycerol and glyceride fatty acids. Synthesis of glyceride fatty acids from glucose reached maximal rates only after several hours of incubation in Krebs-Henseleit bicarbonate buffer, with or without added bovine albumin. Conversion of glucose to glyceri...
متن کاملInsulin-receptor interaction in isolated fat cells.
Suspensions of fat cells were prepared from rat epididymal adipose tissue by digestion of the tissue with crude bacterial collagenase. One portion of each suspension was exposed to p-chloromercuribenzenesulfonic acid (CMS), washed, and then incubated in Krebs-Ringer-bicarbonate buffer. A second portion, the control, was not exposed to CMS, but it was otherwise treated identically. Acceleration ...
متن کاملPhospholipid Synthesis in Isolated Fat Cells
Diacylglycerol cholinephosphotransferase (EC 2.7.8.2) and diacylglycerol ethanolaminephosphotransferase (EC 2.7.8.1) activities were investigated in microsomes from isolated rat fat cells. Assays based on the conversion of CDP[‘“Clcholine or CDP-[“Clethanolamine to phosphatidylcholine or phosphatidylethanolamine utilized ethanol-dispersed diacylglycerols and 1 to 5 pg of protein. Cholinephospho...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1974
ISSN: 0264-6021
DOI: 10.1042/bj1420477